rabbit polyclonal antibody against ep3  (Cayman Chemical)

Journal: Nature neuroscience

Article Title: NG2 glia protect against prion neurotoxicity by inhibiting microglia-to-neuron prostaglandin E2 signaling.

doi: 10.1038/s41593-024-01663-x

Figure Lengend Snippet: Fig. 7 | PGE2 enhances prion neurotoxicity mainly through the EP4 receptor (Ptger4). a,b, Live-cell imaging (a) and quantitative analysis (b) of chronically prion-infected HovS cells expressing control (Ctrl) transgene or one of the four PGE2 receptors (Ptger1–4). Effects of PGE2 treatment on prion-induced cell toxicity were measured with the ratio of GFP signals under the PGE2 condition against the DMSO condition; n = 4 independent experiments. Data are presented as mean ± s.e.m. One-way ANOVA with Benjamini–Hochberg FDR adjustment for multiple comparisons: P < 0.0001 (Ptger1 versus Ctrl); P = 0.2246 (Ptger2 versus Ctrl); P = 0.3351 (Ptger3 versus Ctrl); P < 0.0001 (Ptger4 versus Ctrl). c, Immunofluorescence of NeuN, Map2 and Tau showing cellular damage of prion- infected primary neurons treated with different concentrations of Ptger4 agonist L902688. d, Quantification of neuronal density as well as Map2-positive and Tau positive areas shown in c; n = 6 independent experiments. Data are presented as

Article Snippet: 3 n atu re p o rtfo lio | rep o rtin g su m m ary A p ril 2 0 2 3 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used mouse monoclonal antibody against actin (1:10,000, Merck Millipore, MAB1501R, clone C4); mouse monoclonal antibody against PrP (POM1, 1:5000, homemade); rabbit polyclonal antibody against NG2 (1:500, MERCK, AB5320); rabbit polyclonal antibody against PDGFRα (1:500, Santa Cruz, SC-338); rabbit monoclonal antibody against NeuN (1:1000, Abcam, ab177487, clone EPR12763); rabbit polyclonal antibody against NG2 (1:500, a gift from Prof. Stallcup); rabbit polyclonal antibody against Iba1 (1:500, Wako, 019-19741); Rat monoclonal antibody against Cd68 (1:200, BioRad, MCA1957, clone FA-11); rabbit polyclonal antibody against Map2 (1:200, Biolegend, 840601), mouse monoclonal antibody against Cox2 (1:200, Santa Cruz, sc-166475, clone D-12); mouse monoclonal antibody against Ptges (1:200, Santa Cruz, sc-365844, clone H-3); rabbit polyclonal antibody against EP1 (1:200, Bioss Antibodies, BS-6316R); rabbit monoclonal antibody against EP2 (1:200, Abcam, ab167171, clone EPR8030(B)); rabbit polyclonal antibody against EP3 (1:200, Cayman Chemical, 101760); mouse monoclonal antibody against EP4 (1:200, ProteinTech, 66921-1-Ig, clone 4A2A12); rat monoclonal antibody against CD11b antibody (30 ul in 10 ml, ThermoFisher Scientific, 14-0112-82, clone M1/70), mouse monoclonal antibody against Tau (1:200, ThermoFisher Scientific, MN1010, clone BT2), chicken polyclonal antibody against NeuN (1:1000, Merck, ABN91), goat polyclonal antibody against rat IgG (30 ul in 10 m, Jackson ImmunoResearch, 112-005-167); HRP-conjugated goat antirabbit IgG antibody (1:10,000, Jackson ImmunoResearch, 111-035-003); HRP-conjugated goat anti-mouse IgG antibody (1:10,000, Jackson ImmunoResearch, 115-035-003); Alexa488-conjugated goat anti-mouse IgG antibody (1:3000, ThermoFisher Scientific, A32723); Alexa594-conjugated goat anti-rabbit IgG antibody (1:3000, ThermoFisher Scientific, A32740); Alexa647-conjugated goat anti-chicken IgG antibody (1:3000, ThermoFisher Scientific, A32933).

Techniques: Live Cell Imaging, Infection, Expressing, Control, Immunofluorescence

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Control, Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322, and GW 627368X on various classes of NHL genes

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques:

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322. and GW 627368X on various classes of NHL genes.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: